Coding

Part:BBa_K786033:Design

Designed by: Kaiyin Feng   Group: iGEM12_Hong_Kong-CUHK   (2012-10-24)


cascade proteins, which consists of casA, casB, casC, casD, casE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BglII site found at 4279
    Illegal BamHI site found at 23
    Illegal BamHI site found at 3224
    Illegal BamHI site found at 3585
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 84
    Illegal PstI site found at 1253
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1764


Design Notes

We amplified the cascade proteins from E.coli K12 genome for further use of our new safety approach.


Source

E.coli K12 genome.

References